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Sarstedt cell culture flask 75 cm 2
Cell Culture Flask 75 Cm 2, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2+flasks/flasks+t75/pmc12547236-49-0-10
Average 86 stars, based on 1 article reviews
cell culture flask 75 cm 2 - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Incubation:

Article Title: Evaluating the Translucency, Surface Roughness, and Cytotoxicity of a PMMA Acrylic Denture Base Reinforced with Bioactive Glasses
Article Snippet: The cells were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium, Sigma-Aldrich, Poznan, Poland) supplemented with 10% FBS (Sigma-Aldrich, Poznan, Poland), Penicillin/Streptomycin (Sigma, Poznan, Poland), and 1% GlutaMAXTM-I (Gibco, New York, NY, USA) in 25 cm 2 flasks (Zeit Buddels, Sarstedt, Germany).

Article Title: Expression Profiling Identified TRPM7 and HER2 as Potential Targets for the Combined Treatment of Cancer Cells
Article Snippet: To study the impact of TRPM7 KO mutations and NS8593 on transcriptome of HAP1 cells, WT, TRPM7 KO-01, and KO-04 cells were maintained in 25 cm 2 flasks (Sarstedt, Nümbrecht, Germany) with IMDM containing 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin (all from Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 mM MgCl 2 (IMDM-Mg).

Article Title: Trehalose Attenuates In Vitro Neurotoxicity of 6-Hydroxydopamine by Reducing Oxidative Stress and Activation of MAPK/AMPK Signaling Pathways
Article Snippet: Cells were incubated in 96-well flat-bottom plates (3 × 10 4 cells/well) for viability assays, 24-well plates (1 × 10 5 cells/well) for flow cytometric analysis, 60 mm Petri dishes (2 × 10 6 cells) for electron microscopy and immunoblotting, and 175 cm 2 flasks (10 × 10 6 cells) for determination of reduced glutathione (all cell culture plastic from Sarstedt AG & Co., Nümbrecht, Germany).

Article Title: A Specific Collagen Hydrolysate Improves Postprandial Glucose Tolerance in Normoglycemic and Prediabetic Mice and in a First Proof of Concept Study in Healthy, Normoglycemic and Prediabetic Humans
Article Snippet: Cells were routinely maintained at 37°C in a 5% CO 2 ‐modified atmosphere in 75 cm 2 flasks (Sarstedt, Nümbrecht, Germany).

Article Title: Double Deletion of EP402R and EP153R in the Attenuated Lv17/WB/Rie1 African Swine Fever Virus (ASFV) Enhances Safety, Provides DIVA Compatibility, and Confers Complete Protection Against a Genotype II Virulent Strain
Article Snippet: For stock preparation, 100 μL of the supernatant of isolated viruses was added to PAMs plated in 10 mL of culture medium on 75 cm 2 flasks (SARSTEDT AG&Co.

Article Title: Besnoitia besnoiti tachyzoite replication in bovine primary endothelial cells relies on host Niemann–Pick type C protein 1 for cholesterol acquisition
Article Snippet: Additionally, to determine the impact of U18666A on BUVEC neutral lipid content, cells were seeded into 25 cm 2 flasks (Sarstedt) and treated with U18666A (7.5 μM) for 48 h. Thereafter, cells were collected, pelleted and stained with BODIPY 493/503 (2 μg/mL; 1 h, 37°C, Cayman) as described elsewhere ( , ), and analyzed by flow cytometry (Accuri C6 ® , Becton-Dickinson).

Article Title: 3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization
Article Snippet: For transduction, NSCs were seeded into 25 cm 2 flasks (Sarstedt AG & Co. KG) at a density of 2 × 10 5 cells per flask.

Article Title: Red Wine Extract Prevents Oxidative Stress and Inflammation in ARPE-19 Retinal Cells
Article Snippet: The ARPE-19 cells were seeded at a density of 7500 cells/cm 2 into 75 cm 2 flasks (Sarstedt, Marnay, France) for the immunoblotting experiments, 12-well plates (Sarstedt, Marnay, France) for flow cytometry, 24-well plates (Sarstedt, Marnay, France) for immunofluorescence, or 48-well plates (Sarstedt, Marnay, France) for the ELISA and DCFDA assays, in DMEM/F12 supplemented with 10% FBS.

Electron Microscopy:

Article Title: Evaluating the Translucency, Surface Roughness, and Cytotoxicity of a PMMA Acrylic Denture Base Reinforced with Bioactive Glasses
Article Snippet: The cells were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium, Sigma-Aldrich, Poznan, Poland) supplemented with 10% FBS (Sigma-Aldrich, Poznan, Poland), Penicillin/Streptomycin (Sigma, Poznan, Poland), and 1% GlutaMAXTM-I (Gibco, New York, NY, USA) in 25 cm 2 flasks (Zeit Buddels, Sarstedt, Germany).

Article Title: Expression Profiling Identified TRPM7 and HER2 as Potential Targets for the Combined Treatment of Cancer Cells
Article Snippet: To study the impact of TRPM7 KO mutations and NS8593 on transcriptome of HAP1 cells, WT, TRPM7 KO-01, and KO-04 cells were maintained in 25 cm 2 flasks (Sarstedt, Nümbrecht, Germany) with IMDM containing 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin (all from Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 mM MgCl 2 (IMDM-Mg).

Article Title: Trehalose Attenuates In Vitro Neurotoxicity of 6-Hydroxydopamine by Reducing Oxidative Stress and Activation of MAPK/AMPK Signaling Pathways
Article Snippet: Cells were incubated in 96-well flat-bottom plates (3 × 10 4 cells/well) for viability assays, 24-well plates (1 × 10 5 cells/well) for flow cytometric analysis, 60 mm Petri dishes (2 × 10 6 cells) for electron microscopy and immunoblotting, and 175 cm 2 flasks (10 × 10 6 cells) for determination of reduced glutathione (all cell culture plastic from Sarstedt AG & Co., Nümbrecht, Germany).

Article Title: A Specific Collagen Hydrolysate Improves Postprandial Glucose Tolerance in Normoglycemic and Prediabetic Mice and in a First Proof of Concept Study in Healthy, Normoglycemic and Prediabetic Humans
Article Snippet: Cells were routinely maintained at 37°C in a 5% CO 2 ‐modified atmosphere in 75 cm 2 flasks (Sarstedt, Nümbrecht, Germany).

Article Title: Double Deletion of EP402R and EP153R in the Attenuated Lv17/WB/Rie1 African Swine Fever Virus (ASFV) Enhances Safety, Provides DIVA Compatibility, and Confers Complete Protection Against a Genotype II Virulent Strain
Article Snippet: For stock preparation, 100 μL of the supernatant of isolated viruses was added to PAMs plated in 10 mL of culture medium on 75 cm 2 flasks (SARSTEDT AG&Co.

Article Title: Besnoitia besnoiti tachyzoite replication in bovine primary endothelial cells relies on host Niemann–Pick type C protein 1 for cholesterol acquisition
Article Snippet: Additionally, to determine the impact of U18666A on BUVEC neutral lipid content, cells were seeded into 25 cm 2 flasks (Sarstedt) and treated with U18666A (7.5 μM) for 48 h. Thereafter, cells were collected, pelleted and stained with BODIPY 493/503 (2 μg/mL; 1 h, 37°C, Cayman) as described elsewhere ( , ), and analyzed by flow cytometry (Accuri C6 ® , Becton-Dickinson).

Article Title: 3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization
Article Snippet: For transduction, NSCs were seeded into 25 cm 2 flasks (Sarstedt AG & Co. KG) at a density of 2 × 10 5 cells per flask.

Article Title: Red Wine Extract Prevents Oxidative Stress and Inflammation in ARPE-19 Retinal Cells
Article Snippet: The ARPE-19 cells were seeded at a density of 7500 cells/cm 2 into 75 cm 2 flasks (Sarstedt, Marnay, France) for the immunoblotting experiments, 12-well plates (Sarstedt, Marnay, France) for flow cytometry, 24-well plates (Sarstedt, Marnay, France) for immunofluorescence, or 48-well plates (Sarstedt, Marnay, France) for the ELISA and DCFDA assays, in DMEM/F12 supplemented with 10% FBS.

Western Blot:

Article Title: Evaluating the Translucency, Surface Roughness, and Cytotoxicity of a PMMA Acrylic Denture Base Reinforced with Bioactive Glasses
Article Snippet: The cells were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium, Sigma-Aldrich, Poznan, Poland) supplemented with 10% FBS (Sigma-Aldrich, Poznan, Poland), Penicillin/Streptomycin (Sigma, Poznan, Poland), and 1% GlutaMAXTM-I (Gibco, New York, NY, USA) in 25 cm 2 flasks (Zeit Buddels, Sarstedt, Germany).

Article Title: Expression Profiling Identified TRPM7 and HER2 as Potential Targets for the Combined Treatment of Cancer Cells
Article Snippet: To study the impact of TRPM7 KO mutations and NS8593 on transcriptome of HAP1 cells, WT, TRPM7 KO-01, and KO-04 cells were maintained in 25 cm 2 flasks (Sarstedt, Nümbrecht, Germany) with IMDM containing 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin (all from Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 mM MgCl 2 (IMDM-Mg).

Article Title: Trehalose Attenuates In Vitro Neurotoxicity of 6-Hydroxydopamine by Reducing Oxidative Stress and Activation of MAPK/AMPK Signaling Pathways
Article Snippet: Cells were incubated in 96-well flat-bottom plates (3 × 10 4 cells/well) for viability assays, 24-well plates (1 × 10 5 cells/well) for flow cytometric analysis, 60 mm Petri dishes (2 × 10 6 cells) for electron microscopy and immunoblotting, and 175 cm 2 flasks (10 × 10 6 cells) for determination of reduced glutathione (all cell culture plastic from Sarstedt AG & Co., Nümbrecht, Germany).

Article Title: A Specific Collagen Hydrolysate Improves Postprandial Glucose Tolerance in Normoglycemic and Prediabetic Mice and in a First Proof of Concept Study in Healthy, Normoglycemic and Prediabetic Humans
Article Snippet: Cells were routinely maintained at 37°C in a 5% CO 2 ‐modified atmosphere in 75 cm 2 flasks (Sarstedt, Nümbrecht, Germany).

Article Title: Double Deletion of EP402R and EP153R in the Attenuated Lv17/WB/Rie1 African Swine Fever Virus (ASFV) Enhances Safety, Provides DIVA Compatibility, and Confers Complete Protection Against a Genotype II Virulent Strain
Article Snippet: For stock preparation, 100 μL of the supernatant of isolated viruses was added to PAMs plated in 10 mL of culture medium on 75 cm 2 flasks (SARSTEDT AG&Co.

Article Title: Besnoitia besnoiti tachyzoite replication in bovine primary endothelial cells relies on host Niemann–Pick type C protein 1 for cholesterol acquisition
Article Snippet: Additionally, to determine the impact of U18666A on BUVEC neutral lipid content, cells were seeded into 25 cm 2 flasks (Sarstedt) and treated with U18666A (7.5 μM) for 48 h. Thereafter, cells were collected, pelleted and stained with BODIPY 493/503 (2 μg/mL; 1 h, 37°C, Cayman) as described elsewhere ( , ), and analyzed by flow cytometry (Accuri C6 ® , Becton-Dickinson).

Article Title: 3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization
Article Snippet: For transduction, NSCs were seeded into 25 cm 2 flasks (Sarstedt AG & Co. KG) at a density of 2 × 10 5 cells per flask.

Article Title: Red Wine Extract Prevents Oxidative Stress and Inflammation in ARPE-19 Retinal Cells
Article Snippet: The ARPE-19 cells were seeded at a density of 7500 cells/cm 2 into 75 cm 2 flasks (Sarstedt, Marnay, France) for the immunoblotting experiments, 12-well plates (Sarstedt, Marnay, France) for flow cytometry, 24-well plates (Sarstedt, Marnay, France) for immunofluorescence, or 48-well plates (Sarstedt, Marnay, France) for the ELISA and DCFDA assays, in DMEM/F12 supplemented with 10% FBS.

Cell Culture:

Article Title: Evaluating the Translucency, Surface Roughness, and Cytotoxicity of a PMMA Acrylic Denture Base Reinforced with Bioactive Glasses
Article Snippet: The cells were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium, Sigma-Aldrich, Poznan, Poland) supplemented with 10% FBS (Sigma-Aldrich, Poznan, Poland), Penicillin/Streptomycin (Sigma, Poznan, Poland), and 1% GlutaMAXTM-I (Gibco, New York, NY, USA) in 25 cm 2 flasks (Zeit Buddels, Sarstedt, Germany).

Article Title: Expression Profiling Identified TRPM7 and HER2 as Potential Targets for the Combined Treatment of Cancer Cells
Article Snippet: To study the impact of TRPM7 KO mutations and NS8593 on transcriptome of HAP1 cells, WT, TRPM7 KO-01, and KO-04 cells were maintained in 25 cm 2 flasks (Sarstedt, Nümbrecht, Germany) with IMDM containing 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin (all from Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 mM MgCl 2 (IMDM-Mg).

Article Title: Trehalose Attenuates In Vitro Neurotoxicity of 6-Hydroxydopamine by Reducing Oxidative Stress and Activation of MAPK/AMPK Signaling Pathways
Article Snippet: Cells were incubated in 96-well flat-bottom plates (3 × 10 4 cells/well) for viability assays, 24-well plates (1 × 10 5 cells/well) for flow cytometric analysis, 60 mm Petri dishes (2 × 10 6 cells) for electron microscopy and immunoblotting, and 175 cm 2 flasks (10 × 10 6 cells) for determination of reduced glutathione (all cell culture plastic from Sarstedt AG & Co., Nümbrecht, Germany).

Article Title: A Specific Collagen Hydrolysate Improves Postprandial Glucose Tolerance in Normoglycemic and Prediabetic Mice and in a First Proof of Concept Study in Healthy, Normoglycemic and Prediabetic Humans
Article Snippet: Cells were routinely maintained at 37°C in a 5% CO 2 ‐modified atmosphere in 75 cm 2 flasks (Sarstedt, Nümbrecht, Germany).

Article Title: Double Deletion of EP402R and EP153R in the Attenuated Lv17/WB/Rie1 African Swine Fever Virus (ASFV) Enhances Safety, Provides DIVA Compatibility, and Confers Complete Protection Against a Genotype II Virulent Strain
Article Snippet: For stock preparation, 100 μL of the supernatant of isolated viruses was added to PAMs plated in 10 mL of culture medium on 75 cm 2 flasks (SARSTEDT AG&Co.

Article Title: Besnoitia besnoiti tachyzoite replication in bovine primary endothelial cells relies on host Niemann–Pick type C protein 1 for cholesterol acquisition
Article Snippet: Additionally, to determine the impact of U18666A on BUVEC neutral lipid content, cells were seeded into 25 cm 2 flasks (Sarstedt) and treated with U18666A (7.5 μM) for 48 h. Thereafter, cells were collected, pelleted and stained with BODIPY 493/503 (2 μg/mL; 1 h, 37°C, Cayman) as described elsewhere ( , ), and analyzed by flow cytometry (Accuri C6 ® , Becton-Dickinson).

Article Title: 3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization
Article Snippet: For transduction, NSCs were seeded into 25 cm 2 flasks (Sarstedt AG & Co. KG) at a density of 2 × 10 5 cells per flask.

Article Title: Red Wine Extract Prevents Oxidative Stress and Inflammation in ARPE-19 Retinal Cells
Article Snippet: The ARPE-19 cells were seeded at a density of 7500 cells/cm 2 into 75 cm 2 flasks (Sarstedt, Marnay, France) for the immunoblotting experiments, 12-well plates (Sarstedt, Marnay, France) for flow cytometry, 24-well plates (Sarstedt, Marnay, France) for immunofluorescence, or 48-well plates (Sarstedt, Marnay, France) for the ELISA and DCFDA assays, in DMEM/F12 supplemented with 10% FBS.

Staining:

Article Title: Evaluating the Translucency, Surface Roughness, and Cytotoxicity of a PMMA Acrylic Denture Base Reinforced with Bioactive Glasses
Article Snippet: The cells were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium, Sigma-Aldrich, Poznan, Poland) supplemented with 10% FBS (Sigma-Aldrich, Poznan, Poland), Penicillin/Streptomycin (Sigma, Poznan, Poland), and 1% GlutaMAXTM-I (Gibco, New York, NY, USA) in 25 cm 2 flasks (Zeit Buddels, Sarstedt, Germany).

Article Title: Expression Profiling Identified TRPM7 and HER2 as Potential Targets for the Combined Treatment of Cancer Cells
Article Snippet: To study the impact of TRPM7 KO mutations and NS8593 on transcriptome of HAP1 cells, WT, TRPM7 KO-01, and KO-04 cells were maintained in 25 cm 2 flasks (Sarstedt, Nümbrecht, Germany) with IMDM containing 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin (all from Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 mM MgCl 2 (IMDM-Mg).

Article Title: Trehalose Attenuates In Vitro Neurotoxicity of 6-Hydroxydopamine by Reducing Oxidative Stress and Activation of MAPK/AMPK Signaling Pathways
Article Snippet: Cells were incubated in 96-well flat-bottom plates (3 × 10 4 cells/well) for viability assays, 24-well plates (1 × 10 5 cells/well) for flow cytometric analysis, 60 mm Petri dishes (2 × 10 6 cells) for electron microscopy and immunoblotting, and 175 cm 2 flasks (10 × 10 6 cells) for determination of reduced glutathione (all cell culture plastic from Sarstedt AG & Co., Nümbrecht, Germany).

Article Title: A Specific Collagen Hydrolysate Improves Postprandial Glucose Tolerance in Normoglycemic and Prediabetic Mice and in a First Proof of Concept Study in Healthy, Normoglycemic and Prediabetic Humans
Article Snippet: Cells were routinely maintained at 37°C in a 5% CO 2 ‐modified atmosphere in 75 cm 2 flasks (Sarstedt, Nümbrecht, Germany).

Article Title: Double Deletion of EP402R and EP153R in the Attenuated Lv17/WB/Rie1 African Swine Fever Virus (ASFV) Enhances Safety, Provides DIVA Compatibility, and Confers Complete Protection Against a Genotype II Virulent Strain
Article Snippet: For stock preparation, 100 μL of the supernatant of isolated viruses was added to PAMs plated in 10 mL of culture medium on 75 cm 2 flasks (SARSTEDT AG&Co.

Article Title: Besnoitia besnoiti tachyzoite replication in bovine primary endothelial cells relies on host Niemann–Pick type C protein 1 for cholesterol acquisition
Article Snippet: Additionally, to determine the impact of U18666A on BUVEC neutral lipid content, cells were seeded into 25 cm 2 flasks (Sarstedt) and treated with U18666A (7.5 μM) for 48 h. Thereafter, cells were collected, pelleted and stained with BODIPY 493/503 (2 μg/mL; 1 h, 37°C, Cayman) as described elsewhere ( , ), and analyzed by flow cytometry (Accuri C6 ® , Becton-Dickinson).

Article Title: 3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization
Article Snippet: For transduction, NSCs were seeded into 25 cm 2 flasks (Sarstedt AG & Co. KG) at a density of 2 × 10 5 cells per flask.

Article Title: Red Wine Extract Prevents Oxidative Stress and Inflammation in ARPE-19 Retinal Cells
Article Snippet: The ARPE-19 cells were seeded at a density of 7500 cells/cm 2 into 75 cm 2 flasks (Sarstedt, Marnay, France) for the immunoblotting experiments, 12-well plates (Sarstedt, Marnay, France) for flow cytometry, 24-well plates (Sarstedt, Marnay, France) for immunofluorescence, or 48-well plates (Sarstedt, Marnay, France) for the ELISA and DCFDA assays, in DMEM/F12 supplemented with 10% FBS.

Flow Cytometry:

Article Title: Evaluating the Translucency, Surface Roughness, and Cytotoxicity of a PMMA Acrylic Denture Base Reinforced with Bioactive Glasses
Article Snippet: The cells were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium, Sigma-Aldrich, Poznan, Poland) supplemented with 10% FBS (Sigma-Aldrich, Poznan, Poland), Penicillin/Streptomycin (Sigma, Poznan, Poland), and 1% GlutaMAXTM-I (Gibco, New York, NY, USA) in 25 cm 2 flasks (Zeit Buddels, Sarstedt, Germany).

Article Title: Expression Profiling Identified TRPM7 and HER2 as Potential Targets for the Combined Treatment of Cancer Cells
Article Snippet: To study the impact of TRPM7 KO mutations and NS8593 on transcriptome of HAP1 cells, WT, TRPM7 KO-01, and KO-04 cells were maintained in 25 cm 2 flasks (Sarstedt, Nümbrecht, Germany) with IMDM containing 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin (all from Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 mM MgCl 2 (IMDM-Mg).

Article Title: Trehalose Attenuates In Vitro Neurotoxicity of 6-Hydroxydopamine by Reducing Oxidative Stress and Activation of MAPK/AMPK Signaling Pathways
Article Snippet: Cells were incubated in 96-well flat-bottom plates (3 × 10 4 cells/well) for viability assays, 24-well plates (1 × 10 5 cells/well) for flow cytometric analysis, 60 mm Petri dishes (2 × 10 6 cells) for electron microscopy and immunoblotting, and 175 cm 2 flasks (10 × 10 6 cells) for determination of reduced glutathione (all cell culture plastic from Sarstedt AG & Co., Nümbrecht, Germany).

Article Title: A Specific Collagen Hydrolysate Improves Postprandial Glucose Tolerance in Normoglycemic and Prediabetic Mice and in a First Proof of Concept Study in Healthy, Normoglycemic and Prediabetic Humans
Article Snippet: Cells were routinely maintained at 37°C in a 5% CO 2 ‐modified atmosphere in 75 cm 2 flasks (Sarstedt, Nümbrecht, Germany).

Article Title: Double Deletion of EP402R and EP153R in the Attenuated Lv17/WB/Rie1 African Swine Fever Virus (ASFV) Enhances Safety, Provides DIVA Compatibility, and Confers Complete Protection Against a Genotype II Virulent Strain
Article Snippet: For stock preparation, 100 μL of the supernatant of isolated viruses was added to PAMs plated in 10 mL of culture medium on 75 cm 2 flasks (SARSTEDT AG&Co.

Article Title: Besnoitia besnoiti tachyzoite replication in bovine primary endothelial cells relies on host Niemann–Pick type C protein 1 for cholesterol acquisition
Article Snippet: Additionally, to determine the impact of U18666A on BUVEC neutral lipid content, cells were seeded into 25 cm 2 flasks (Sarstedt) and treated with U18666A (7.5 μM) for 48 h. Thereafter, cells were collected, pelleted and stained with BODIPY 493/503 (2 μg/mL; 1 h, 37°C, Cayman) as described elsewhere ( , ), and analyzed by flow cytometry (Accuri C6 ® , Becton-Dickinson).

Article Title: 3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization
Article Snippet: For transduction, NSCs were seeded into 25 cm 2 flasks (Sarstedt AG & Co. KG) at a density of 2 × 10 5 cells per flask.

Article Title: Red Wine Extract Prevents Oxidative Stress and Inflammation in ARPE-19 Retinal Cells
Article Snippet: The ARPE-19 cells were seeded at a density of 7500 cells/cm 2 into 75 cm 2 flasks (Sarstedt, Marnay, France) for the immunoblotting experiments, 12-well plates (Sarstedt, Marnay, France) for flow cytometry, 24-well plates (Sarstedt, Marnay, France) for immunofluorescence, or 48-well plates (Sarstedt, Marnay, France) for the ELISA and DCFDA assays, in DMEM/F12 supplemented with 10% FBS.

Modification:

Article Title: Evaluating the Translucency, Surface Roughness, and Cytotoxicity of a PMMA Acrylic Denture Base Reinforced with Bioactive Glasses
Article Snippet: The cells were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium, Sigma-Aldrich, Poznan, Poland) supplemented with 10% FBS (Sigma-Aldrich, Poznan, Poland), Penicillin/Streptomycin (Sigma, Poznan, Poland), and 1% GlutaMAXTM-I (Gibco, New York, NY, USA) in 25 cm 2 flasks (Zeit Buddels, Sarstedt, Germany).

Article Title: Expression Profiling Identified TRPM7 and HER2 as Potential Targets for the Combined Treatment of Cancer Cells
Article Snippet: To study the impact of TRPM7 KO mutations and NS8593 on transcriptome of HAP1 cells, WT, TRPM7 KO-01, and KO-04 cells were maintained in 25 cm 2 flasks (Sarstedt, Nümbrecht, Germany) with IMDM containing 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin (all from Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 mM MgCl 2 (IMDM-Mg).

Article Title: Trehalose Attenuates In Vitro Neurotoxicity of 6-Hydroxydopamine by Reducing Oxidative Stress and Activation of MAPK/AMPK Signaling Pathways
Article Snippet: Cells were incubated in 96-well flat-bottom plates (3 × 10 4 cells/well) for viability assays, 24-well plates (1 × 10 5 cells/well) for flow cytometric analysis, 60 mm Petri dishes (2 × 10 6 cells) for electron microscopy and immunoblotting, and 175 cm 2 flasks (10 × 10 6 cells) for determination of reduced glutathione (all cell culture plastic from Sarstedt AG & Co., Nümbrecht, Germany).

Article Title: A Specific Collagen Hydrolysate Improves Postprandial Glucose Tolerance in Normoglycemic and Prediabetic Mice and in a First Proof of Concept Study in Healthy, Normoglycemic and Prediabetic Humans
Article Snippet: Cells were routinely maintained at 37°C in a 5% CO 2 ‐modified atmosphere in 75 cm 2 flasks (Sarstedt, Nümbrecht, Germany).

Article Title: Double Deletion of EP402R and EP153R in the Attenuated Lv17/WB/Rie1 African Swine Fever Virus (ASFV) Enhances Safety, Provides DIVA Compatibility, and Confers Complete Protection Against a Genotype II Virulent Strain
Article Snippet: For stock preparation, 100 μL of the supernatant of isolated viruses was added to PAMs plated in 10 mL of culture medium on 75 cm 2 flasks (SARSTEDT AG&Co.

Article Title: Besnoitia besnoiti tachyzoite replication in bovine primary endothelial cells relies on host Niemann–Pick type C protein 1 for cholesterol acquisition
Article Snippet: Additionally, to determine the impact of U18666A on BUVEC neutral lipid content, cells were seeded into 25 cm 2 flasks (Sarstedt) and treated with U18666A (7.5 μM) for 48 h. Thereafter, cells were collected, pelleted and stained with BODIPY 493/503 (2 μg/mL; 1 h, 37°C, Cayman) as described elsewhere ( , ), and analyzed by flow cytometry (Accuri C6 ® , Becton-Dickinson).

Article Title: 3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization
Article Snippet: For transduction, NSCs were seeded into 25 cm 2 flasks (Sarstedt AG & Co. KG) at a density of 2 × 10 5 cells per flask.

Article Title: Red Wine Extract Prevents Oxidative Stress and Inflammation in ARPE-19 Retinal Cells
Article Snippet: The ARPE-19 cells were seeded at a density of 7500 cells/cm 2 into 75 cm 2 flasks (Sarstedt, Marnay, France) for the immunoblotting experiments, 12-well plates (Sarstedt, Marnay, France) for flow cytometry, 24-well plates (Sarstedt, Marnay, France) for immunofluorescence, or 48-well plates (Sarstedt, Marnay, France) for the ELISA and DCFDA assays, in DMEM/F12 supplemented with 10% FBS.

Immunofluorescence:

Article Title: Evaluating the Translucency, Surface Roughness, and Cytotoxicity of a PMMA Acrylic Denture Base Reinforced with Bioactive Glasses
Article Snippet: The cells were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium, Sigma-Aldrich, Poznan, Poland) supplemented with 10% FBS (Sigma-Aldrich, Poznan, Poland), Penicillin/Streptomycin (Sigma, Poznan, Poland), and 1% GlutaMAXTM-I (Gibco, New York, NY, USA) in 25 cm 2 flasks (Zeit Buddels, Sarstedt, Germany).

Article Title: Expression Profiling Identified TRPM7 and HER2 as Potential Targets for the Combined Treatment of Cancer Cells
Article Snippet: To study the impact of TRPM7 KO mutations and NS8593 on transcriptome of HAP1 cells, WT, TRPM7 KO-01, and KO-04 cells were maintained in 25 cm 2 flasks (Sarstedt, Nümbrecht, Germany) with IMDM containing 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin (all from Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 mM MgCl 2 (IMDM-Mg).

Article Title: Trehalose Attenuates In Vitro Neurotoxicity of 6-Hydroxydopamine by Reducing Oxidative Stress and Activation of MAPK/AMPK Signaling Pathways
Article Snippet: Cells were incubated in 96-well flat-bottom plates (3 × 10 4 cells/well) for viability assays, 24-well plates (1 × 10 5 cells/well) for flow cytometric analysis, 60 mm Petri dishes (2 × 10 6 cells) for electron microscopy and immunoblotting, and 175 cm 2 flasks (10 × 10 6 cells) for determination of reduced glutathione (all cell culture plastic from Sarstedt AG & Co., Nümbrecht, Germany).

Article Title: A Specific Collagen Hydrolysate Improves Postprandial Glucose Tolerance in Normoglycemic and Prediabetic Mice and in a First Proof of Concept Study in Healthy, Normoglycemic and Prediabetic Humans
Article Snippet: Cells were routinely maintained at 37°C in a 5% CO 2 ‐modified atmosphere in 75 cm 2 flasks (Sarstedt, Nümbrecht, Germany).

Article Title: Double Deletion of EP402R and EP153R in the Attenuated Lv17/WB/Rie1 African Swine Fever Virus (ASFV) Enhances Safety, Provides DIVA Compatibility, and Confers Complete Protection Against a Genotype II Virulent Strain
Article Snippet: For stock preparation, 100 μL of the supernatant of isolated viruses was added to PAMs plated in 10 mL of culture medium on 75 cm 2 flasks (SARSTEDT AG&Co.

Article Title: Besnoitia besnoiti tachyzoite replication in bovine primary endothelial cells relies on host Niemann–Pick type C protein 1 for cholesterol acquisition
Article Snippet: Additionally, to determine the impact of U18666A on BUVEC neutral lipid content, cells were seeded into 25 cm 2 flasks (Sarstedt) and treated with U18666A (7.5 μM) for 48 h. Thereafter, cells were collected, pelleted and stained with BODIPY 493/503 (2 μg/mL; 1 h, 37°C, Cayman) as described elsewhere ( , ), and analyzed by flow cytometry (Accuri C6 ® , Becton-Dickinson).

Article Title: 3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization
Article Snippet: For transduction, NSCs were seeded into 25 cm 2 flasks (Sarstedt AG & Co. KG) at a density of 2 × 10 5 cells per flask.

Article Title: Red Wine Extract Prevents Oxidative Stress and Inflammation in ARPE-19 Retinal Cells
Article Snippet: The ARPE-19 cells were seeded at a density of 7500 cells/cm 2 into 75 cm 2 flasks (Sarstedt, Marnay, France) for the immunoblotting experiments, 12-well plates (Sarstedt, Marnay, France) for flow cytometry, 24-well plates (Sarstedt, Marnay, France) for immunofluorescence, or 48-well plates (Sarstedt, Marnay, France) for the ELISA and DCFDA assays, in DMEM/F12 supplemented with 10% FBS.

Enzyme-linked Immunosorbent Assay:

Article Title: Evaluating the Translucency, Surface Roughness, and Cytotoxicity of a PMMA Acrylic Denture Base Reinforced with Bioactive Glasses
Article Snippet: The cells were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium, Sigma-Aldrich, Poznan, Poland) supplemented with 10% FBS (Sigma-Aldrich, Poznan, Poland), Penicillin/Streptomycin (Sigma, Poznan, Poland), and 1% GlutaMAXTM-I (Gibco, New York, NY, USA) in 25 cm 2 flasks (Zeit Buddels, Sarstedt, Germany).

Article Title: Expression Profiling Identified TRPM7 and HER2 as Potential Targets for the Combined Treatment of Cancer Cells
Article Snippet: To study the impact of TRPM7 KO mutations and NS8593 on transcriptome of HAP1 cells, WT, TRPM7 KO-01, and KO-04 cells were maintained in 25 cm 2 flasks (Sarstedt, Nümbrecht, Germany) with IMDM containing 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin (all from Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 mM MgCl 2 (IMDM-Mg).

Article Title: Trehalose Attenuates In Vitro Neurotoxicity of 6-Hydroxydopamine by Reducing Oxidative Stress and Activation of MAPK/AMPK Signaling Pathways
Article Snippet: Cells were incubated in 96-well flat-bottom plates (3 × 10 4 cells/well) for viability assays, 24-well plates (1 × 10 5 cells/well) for flow cytometric analysis, 60 mm Petri dishes (2 × 10 6 cells) for electron microscopy and immunoblotting, and 175 cm 2 flasks (10 × 10 6 cells) for determination of reduced glutathione (all cell culture plastic from Sarstedt AG & Co., Nümbrecht, Germany).

Article Title: A Specific Collagen Hydrolysate Improves Postprandial Glucose Tolerance in Normoglycemic and Prediabetic Mice and in a First Proof of Concept Study in Healthy, Normoglycemic and Prediabetic Humans
Article Snippet: Cells were routinely maintained at 37°C in a 5% CO 2 ‐modified atmosphere in 75 cm 2 flasks (Sarstedt, Nümbrecht, Germany).

Article Title: Double Deletion of EP402R and EP153R in the Attenuated Lv17/WB/Rie1 African Swine Fever Virus (ASFV) Enhances Safety, Provides DIVA Compatibility, and Confers Complete Protection Against a Genotype II Virulent Strain
Article Snippet: For stock preparation, 100 μL of the supernatant of isolated viruses was added to PAMs plated in 10 mL of culture medium on 75 cm 2 flasks (SARSTEDT AG&Co.

Article Title: Besnoitia besnoiti tachyzoite replication in bovine primary endothelial cells relies on host Niemann–Pick type C protein 1 for cholesterol acquisition
Article Snippet: Additionally, to determine the impact of U18666A on BUVEC neutral lipid content, cells were seeded into 25 cm 2 flasks (Sarstedt) and treated with U18666A (7.5 μM) for 48 h. Thereafter, cells were collected, pelleted and stained with BODIPY 493/503 (2 μg/mL; 1 h, 37°C, Cayman) as described elsewhere ( , ), and analyzed by flow cytometry (Accuri C6 ® , Becton-Dickinson).

Article Title: 3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization
Article Snippet: For transduction, NSCs were seeded into 25 cm 2 flasks (Sarstedt AG & Co. KG) at a density of 2 × 10 5 cells per flask.

Article Title: Red Wine Extract Prevents Oxidative Stress and Inflammation in ARPE-19 Retinal Cells
Article Snippet: The ARPE-19 cells were seeded at a density of 7500 cells/cm 2 into 75 cm 2 flasks (Sarstedt, Marnay, France) for the immunoblotting experiments, 12-well plates (Sarstedt, Marnay, France) for flow cytometry, 24-well plates (Sarstedt, Marnay, France) for immunofluorescence, or 48-well plates (Sarstedt, Marnay, France) for the ELISA and DCFDA assays, in DMEM/F12 supplemented with 10% FBS.



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